BMMC Cytospin Calculator

Femoral & Calvarial Mast Cell Cultures

Funnel: Fisherbrand™ Single Cytology Funnel
Catalog: 10-355
Maximum sample volume: 400 µL

Example: Enter 0.5 for 500,000 cells/mL.

Cytospin Preparation

Viable cell concentration: —

Pipette the following volumes into the cytospin funnel:

Add Cell Suspension to Cytospin Funnel (µL) —
Add Diluent to Cytospin Funnel (µL) —
Final Funnel Volume: —

Calculations are based on viable cell counts. The 100 µL minimum working volume is a proposed preparation standard; confirm appropriate loading volume and slide quality for your cytospin instrument. Do not exceed the 400 µL funnel capacity.

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